Blar i NORCE vitenarkiv på forfatter "Subburaj, Saminathan"
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A DNA-Free Editing Platform for Genetic Screens in Soybean via CRISPR/Cas9 Ribonucleoprotein Delivery
Subburaj, Saminathan; Zanatta, Caroline Bedin; Nunn, Jennifer Ann Lillebo; Hoepers, Aline Martins; Nodari, Rubens Onofre; Agapito-Tenfen, Sarah Zanon (Journal article; Peer reviewed, 2022)CRISPR/Cas9-based ribonucleoprotein (RNP)-mediated system has the property of minimizing the effects related to the unwanted introduction of vector DNA and random integration of recombinant DNA. Here, we describe a platform ... -
Establishment of targeted mutagenesis in soybean protoplasts using CRISPR/Cas9 RNP delivery via electro−transfection
Subburaj, Saminathan; Agapito-Tenfen, Sarah Zanon (Peer reviewed; Journal article, 2023)The soybean (Glycine max L.) is an important crop with high agronomic value. The improvement of agronomic traits through gene editing techniques has broad application prospects in soybean. The polyethylene glycol (PEG)-mediated ...